FIGURE 2.

Subcellular localization of CLU constructs in HeLa cells. A, Western blot of nuclear and/or cytoplasmic protein of different CLU constructs: psCLU, ΔCLU, β-chain, and α-chain. Control and EmT correspond to pFLAG-BAP and empty vector transfection, respectively. Lamin B1 and α-tubulin were used as control for nuclear and cytoplasmic protein fractions. MW, molecular weight standards. B, confocal microscopy observation of HeLa cells transfected with psCLU-pFLAG, α-chain pFLAG, β-chain pFLAG, and ΔCLU-pFLAG. Control corresponds to nontransfected cells. The cells were incubated with mouse anti-FLAG antibody-labeled FITC (green) to detect the presence of different constructs and with DAPI (blue) to stain the nucleus. The white color corresponds to the superposition (Merge) of FITC and DAPI fluorescence.