FIGURE 3.

Sedimentation assay of mutant actins. Each purified mutant actin was allowed to polymerize under three conditions and then ultracentrifuged. Condition 1, polymerization of actin alone. Condition 2, polymerization with phalloidin. Condition 3, polymerization with additional equimolar concentration of WT actin. The resulting supernatant (S) and pellet (P) fractions were analyzed using 10% PAGE and stained with Coomassie Brilliant Blue (A). The band intensities were then quantified using ImageJ software (B). White, gray, and black bars represent the data obtained under conditions 1, 2 and 3, respectively. Error bars indicate S.D. (n = 3).