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Effects of mutant actin on the polymerization kinetics of WT actin. 1 μm WT actin (15% pyrene-labeled) was mixed with 1 μm WT or mutant actin, after which polymerization was initiated by adding 100 mm KCl and 2 mm MgCl2 at 23 °C. Polymerization was followed for 60–90 min by monitoring the pyrene fluorescence intensity.
