FIGURE 9.

Effects of E226K mutation on Tm·Tn function. Velocities of moving filaments (A) and percentage of filaments moving at uniform speeds (B) of RhPh-labeled WT, E226K filaments, and cofilaments over HMM surfaces were measured in the presence of Tm·Tn and analyzed as in Fig. 8. The differences of the velocities and percentages of filaments moving at uniform speeds between cofilaments and WT filaments at pCa 6.7 were significant (p < 0.05, t test). The velocities (C) and percentages of filaments moving at uniform speeds (D) in the presence or absence of regulatory proteins shown in the bar graphs are those at pCa 5, except that the data in the presence of Tm alone was obtained in the absence of Ca²⁺.