TABLE 3.

Hill parameters for WT and E226K actin filaments

Each parameter ± S.E. was determined by fitting the data presented in Fig. 9 to the Hill equation. The WT data shown here is different from the one in Table 2, and this presumably reflects a minor difference in the experimental conditions (e.g. the Ca-EGTA buffer and the HMM batch).

	Velocity		Filaments moving at uniform speeds
	WT	Cofilament	WT	Cofilament
Hill coefficient	1.9 ± 0.80	1.3 ± 0.07	2.1 ± 0.58	2.9 ± 0.68
pCa50	7.0 ± 0.11	6.9 ± 0.02	7.0 ± 0.06	6.8 ± 0.04
Vmax (μm/s)	4.6 ± 0.40	4.0 ± 0.05
Fmax (%)			84 ± 4.4	79 ± 3.6