TABLE 2.
Activity of ncs1 promoter monitored by luciferase reporter assay
| Condition or media additive | Relative promoter activity |
|---|---|
| WT (no added Ca2+)a | 1 |
| WT (no added Ca2+)a + FK506 | 1.9 |
| WT (0.1 m CaCl2)a | 35.9 |
| WT (0.1 m CaCl2)a + FK506 | 3.2 |
| WT (no added Ca2+)b | 1 |
| WT (0.1 m CaCl2)b | 7.9 |
| ncs1Δ (no added Ca2+)b | 1 |
| ncs1Δ (0.1 m CaCl2)b | 27 |
| prz1− (no added Ca2+)b | 1 |
| prz1− (0.1 m CaCl2)b | 1.8 |
a The ncs1 promoter activity was measured 18 h after treating cells with 0.1 m CaCl2 and/or 1 μg/ml FK506 using bacterial luciferase reporter assay as described in Fig. 2.
b The ncs1 promoter activity was measured 4 h after treating cells with 0.1 m CaCl2.