FIGURE 3.

Effects of miRNAs on the exogenous HNF4α expression in HEK293 cells. The HNF4α expression plasmids including and excluding 3′-UTR used in this study are shown. These plasmids were transfected with HA-tagged GFP expression plasmid and pre-miR miRNA precursor molecules into HEK293 cells. After 48 h, whole cell lysates were prepared. The exogenously expressed HNF4α and HA-tagged GFP protein levels were determined by Western blot analyses. The data represent HNF4α protein level normalized with HA-tagged GFP level relative to that of no transfection (−). Each column represents the mean ± S.D. of three independent experiments. *, p < 0.05; ***, p < 0.001.