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. 2009 Dec 14;285(7):4432–4440. doi: 10.1074/jbc.M109.062216

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FIGURE 1. — Alternate transcriptional starts of HuR mRNA. A, effects of thapsigargin treatment on HuR mRNA expression were assessed by RPA. Lane 1, molecular size markers; lane 2, probe + yeast tRNA, undigested; lane 3, probe + yeast tRNA, digested; lane 4, mRNA from untreated cells; lane 5, mRNA from cells treated with thapsigargin for 1 h; lane 6, mRNA from cells treated with thapsigargin for 1 h and allowed to recover for 6 h. B, effects of ATP depletion/recovery on HuR mRNA as assessed by RPA. Lane 1, mRNA from untreated cells; lane 2, mRNA from cells ATP depleted for 2 h and allowed to recover for 6 h. C, graphical representation of increases in the smaller HuR transcript relative to the longer mRNA, as demonstrated by RPA analysis.