FIGURE 2.

Binding of p65 increases in response to PDGF stimulation or GSK-3 inhibition. Quiescent T98G cells were either untreated or treated for 30 min with PDGF (A) or treated for 60 min with either DMSO or SB-216763 (B). Chromatin fragments were precipitated with either anti-p65 antibody or nonimmune IgG and quantified by real time PCR. The values for nonimmune IgG are from treated cells. The data are presented as the percentage of input and are the means of three independent experiments, ± S.E. In B, * represents sites that showed >1.8-fold increase in binding upon SB-216763 treatment, in addition to statistically significant binding (p < 0.01) as compared with both β-globin and to DMSO control samples, as assessed by t test.