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Inhibition of gene induction by p65 siRNA. T98G cells were transfected with p65 siRNA or a nonspecific control siRNA for 24 h and serum-starved for 48 h to induce quiescence. Immunoblots were used to measure p65 knockdown (A). Gene induction after treatment for 60 min with either DMSO or SB-216763 was measured by real time RT-PCR (B). The data represent the fold increase compared with the vehicle control and are the means of three independent experiments ± S.E.
