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. 2009 Dec 17;285(7):4637–4644. doi: 10.1074/jbc.M109.068007

FIGURE 1.

FIGURE 1.

p65 expression in aP2-p65 mice. A, p65 mRNA in epididymal fat and liver. B, p65 protein in fat and liver. The p65 protein was determined in the whole cell lysate in a Western blot. C, mRNA of NF-κB target genes in epididymal fat of 8-week-old mice. mRNA was determined by quantitative RT-PCR. D, mRNA of p65 and NF-κB target genes in differentiated MEFs of aP2-p65 mice. MEFs were made from embryos of aP2-p65 mice and differentiated into adipocytes in vitro. E, DNA binding activity of NF-κB in differentiated MEFs. The electrophoretic mobility shift assay (EMSA) result is shown. F, mRNA of p65 and NF-κB target genes in differentiated preadipocytes of epididymal fat pad. In the bar graph, values are the mean ± S.E. (n = 3). *, p < 0.05; **, p < 0.001 by Student's t test.