Skip to main content
. 2009 Dec 17;285(7):4637–4644. doi: 10.1074/jbc.M109.068007

FIGURE 3.

FIGURE 3.

Adipose tissue inflammation. Fat tissue was collected at the age of 20 weeks and used in morphology and gene expression studies. A, epididymal fat pad. B, F4/80 immunostaining in WAT. The macrophages are shown in brown. C, adipocyte-specific genes. mRNA expression was determined by real time RT-PCR and expressed as -fold change. D, fatty acid genes, including genes related to fat synthesis and lipolysis. E, inflammatory genes. F, cytokines in serum. TNF-α and IL-6 were determined in serum using multiplex kits. G, macrophage markers. The mRNA expression was determined by real time RT-PCR. In this figure, values are means ± S.E. (n = 6). *, p < 0.05; **, p < 0.001 by Student's t test. Acdc, adiponectin; FAS, fatty acid synthase; SREBP, sterol response element-binding protein; HSL, hormone-sensitive lipase; LPL, lipoprotein lipase.