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. 2003 Nov 12;100(24):14421–14426. doi: 10.1073/pnas.2336152100

Fig. 5.

Fig. 5.

Rho GTPases Rac1 and Cdc42 are activated after stimulation with NT3. The activities of Rho GTPases were measured in rat Schwann cells (A–E) and Cos-7 cells cotransfected with RhoA (F), Rac1 (G and I), or Cdc42 (H and J), together with TrkC. (A–C) The activities of endogenous Rho GTPases were measured after the addition of NT3 with the pull-down assay by using recombinant mDia1-RBD or αPak-CRIB. (D and E) Cells were pretreated with K252a, and the activities of endogenous Rac1 and Cdc42 were measured after the addition of NT3. (F–H) The activities of transfected Rho GTPases were measured after the addition of NT3. (I and J) Cells were pretreated with K252a, and the activities of transfected Rac1 and Cdc42 were measured after the addition of NT3. The total Rho GTPases in the cell lysates were immunoblotted with anti-Rho GTPases antibodies.