FIGURE 1.

All major families of ATP-dependent chromatin remodelers are bound to H2A.Z-containing chromatin. Western blots on purified H2A and H2A.Z chromatin were performed for ATP-dependent core motor proteins (A) and other remodeling complex subunits (B). The primary antibody used in each blot is indicated on the left. A Western blot against the affinity tag on the histone (FLAG) was used as a loading control. Changes in amount of protein present in the input of the purifications were not evident between HeLa, H2A-FLAG, or H2A.Z-FLAG nuclear extracts as determined by Western blot (not shown). The two lower bands observed for Mi-2α migrate at the expected molecular weight for the protein and likely reflect alternatively modified forms of the protein. The top slower migrating species observed only with H2A.Z is >100 kDa larger than expected and is of unknown identity.