FIGURE 2.

Human ACF complex remodels H2A.Z-containing nucleosomes more rapidly than H2A nucleosomes. Cy5 end-labeled recombinant canonical nucleosomes (red) were mixed in equal proportion (30 nm) to Cy3 end-labeled recombinant H2A.Z nucleosomes (green) and remodeled in the presence of 2 mm ATP by the enzyme complex indicated on the left. Remodeling is inferred by digestion with Pst1. The reactions were stopped at the indicated time points, proteinase-digested, and resolved on a 6% acrylamide gel before scanning on a Typhoon imager. Time points from independent reactions with H2A-Cy5 and H2A.Z-Cy3 nucleosomes alone are shown first and indicate that both preparations of nucleosomes are competent for remodeling. The bottom panel is a replicate of ACF remodeling shown in the top panel but done with half the total concentration of each nucleosome (15 nm).