FIGURE 5.
H2A.Z stimulation of remodeling is shared with multiple ISWI complexes regardless of degree of in vivo enrichment. A, colloidal blue staining of recombinant ISWI complexes. B, summary of REA remodeling reactions for H2A.Z enriched SNF2H complex (NoRC) and both H2A.Z depleted complexes containing SNF2H (WICH) and SNF2L (RSF/L). The ratio of kobs of either H2A.Z or H2A.Z-NK nucleosomes to kobs for canonical nucleosomes is graphed for each enzyme complex. The error bars reflect standard deviation from the average of at least seven reactions on multiple nucleosome assemblies. C, REA assay with Cy5 end-labeled recombinant canonical nucleosomes (red) and Cy3 end-labeled recombinant H2A.Z nucleosomes (green) were performed as in Fig. 2.