FIGURE 5.

Vaccinia virus infection disrupts the Bak·Mcl-1 complex. A, HEK 293T cells were mock-infected or infected with VV, VVFLAG-F1L, or VVΔF1L at an m.o.i. of 10 for 12 h before immunoprecipitation (IP) with anti-Mcl-1. Thirty percent of the immunoprecipitates and lysates were Western-blotted (WB) with anti-Bak and anti-Mcl-1. B, HEK 293T cells were infected with VV or VVΔF1L for 3, 6, or 12 h before immunoprecipitation and Western blotting as in A. C, HeLa cells were treated with 2 μm STS for 1, 2, or 3 h to induce apoptosis before immunoprecipitation as in A. Fifty percent of the immunoprecipitates and lysates were Western-blotted with anti-Bak, and 30% were Western-blotted with anti-Mcl-1.