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The cytoplasmic domain of band 3 binds to protein 4.2 but does not interfere with the spectrin-4.2 interaction. A, 125I-labeled CDB3 (0–2.4 μm) was incubated with 1 μm partially purified human protein 4.2, and the complex was immunoprecipitated with an anti-4.2 antibody and counted. B, shown is binding of 35S-labeled protein 4.2 to the GST-α18–21EF fragment of spectrin in the absence or presence of 1 μm CDB3. The data shown are the average of two experiments.
