TABLE 1.

Recombinant proteins

Construct	Molecular mass	Molecular mass without GST	Nucleotides	Amino acids	Vector	Cleavage enzyme
	Da	Da
GST-αEF	45,182	18,197	6970–7440	2262–2418	pGEX-6p	PreScissiona
GST-αEFΔ13	43,653	16,668	6970–7402	2262–2405	pGEX-6p	PreScission
GST-α18–21EF	97,615	70,630	5603–7440	1806–2418	pGEX-6p	PreScission
GST-α18–21EFΔ13	96,090	69,105	5603–7402	1806–2405	pGEX-6p	PreScission
GST-βABD1–4	113,380	86,396	96–2324	1–743	pGEX-2T	Thrombin
GST-4.2G3	36,208	9,223	1690–1935	410–492	pGEX-6p	PreScission

^a PreScission protease (Amersham Biosciences).