FIGURE 5.

SPR analyses of the shFcRn^WT and smFcRn^WT interaction with HSA and MSA. Representative sensorgrams of serial dilutions of MSA injected over immobilized smFcRn^WT at pH 6.0 (A) and 7.4 (B), serial dilutions of HSA injected over immobilized smFcRn^WT at pH 6.0 (C), and serial dilutions of MSA injected to immobilized shFcRn^WT at pH 6.0 (D). In all experiments shFcRn^WT and smFcRn^WT were immobilized by amine coupling to ∼500–800 RU. Dilutions of MSA and HSA were injected over an immobilized receptor at 25 °C. The flow rate was 50 μl/min.