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. 2009 Dec 14;285(7):4826–4836. doi: 10.1074/jbc.M109.081828

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FIGURE 6. — Competitive FcRn-albumin binding across species. A, serial dilutions of HSA (0.5–0.05 μm) and MSA (0.5–0.05 μm) were preincubated with shFcRn^WT (0.05 μm) and injected over immobilized HSA (∼2600 RU). B, serial dilutions of HSA (10.0–0.1 μm) and MSA (1.0–0.1 μm) were preincubated with smFcRn^WT (0.10 μm) and injected over immobilized MSA (∼2000 RU). The representative binding data are presented as percent inhibition of the FcRn binding to immobilized albumin. Injections were performed at 25 °C, and the flow rate was 50 μl/min.