FIGURE 9.

SPR analyses of the interaction of shFcRn^WT and smFcRn^WT with human and mouse ligands. A, HSA, hIgG1, MSA, and mIgG1 injected over shFcRn^WT at pH 6.0. B, MSA, mIgG1, HSA, and hIgG1 injected over shFcRn^WT at pH 6.0. HSA was injected at 10 μm, MSA at 5 μm, and both IgG variants at 100 nm. In all experiments shFcRn^WT and smFcRn^WT were immobilized by amine coupling to ∼600 RU. Injections were performed at 25 °C and the flow rate was 50 μl/min.