FIGURE 3.

ApoER2 and VLDLR internalize Reelin via clathrin-mediated endocytosis. A, 3T3 cells expressing either ApoER2 or VLDLR were incubated with RCM for 1 h, and CCVs were prepared from total cell lysates (CL) as described under “Experimental Procedures.” The presence of Reelin and the respective receptors was analyzed by Western blotting using the appropriate antibodies and the corresponding HRP-coupled secondary antibodies. Clathrin heavy chain was detected as control for enrichment of CCVs. B, CCVs were prepared from primary rat neurons and analyzed as described above.