Figure 1.

Expression of rFII and hFII in HEK293 cells. Cell cultures were transfected with pcDNA3-RFII or pcDNA3-HFII, and after 24 h cells were metabolically labeled with [³⁵S]methionine (100 μCi/ml) in the presence of vitamin K (vit K) or warfarin (warf) for 24 h. Media (M) or lysates (L) were immunoprecipitated, electrophoresed, and detected as described in Materials and Methods. Under-γ-carboxylated prothrombin was defined as prothrombin remaining in solution after BaSO₄ adsorption (lanes 5 and 6). The position of an 84-kDa molecular mass standard is indicated.