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Effect of vitamin K or warfarin on synthesis of prothrombin chimeras in HepG2 cells. HepG2 cells were transfected with pcDNA3-FIIHRR, FIIHHR, FIIRRH, FIIHRH, and FIIRHR, and prothrombin expression was analyzed as described in Fig. 1. A specific rabbit polyclonal antibody to rFII that has no detectable cross-reactivity to hFII(P) was used. The position of an 84-kDa molecular mass standard is indicated.
