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. 2009 Dec 3;285(7):4975–4982. doi: 10.1074/jbc.M109.035105

FIGURE 4.

FIGURE 4.

Osteoprogenitor cell-specific expression of Taf4b in mouse embryos and reduced Taf4b expression in the maxillary process of Tgfbr2fl/fl;Wnt1-Cre mice. A and B, quantitative RT-PCR analyses of indicated genes in the maxilla of Tgfbr2fl/fl (open columns) and Tgfbr2fl/fl;Wnt1-Cre (closed columns) mice at E11.5 (A) and E12.5 (B). Wt, wild type. C–H, whole-mount in situ hybridization of Taf4b in Tgfbr2fl/fl and Tgfbr2fl/fl;Wnt1-Cre mice at E10.5, E11.5, and E12.5. Taf4b mRNA was strongly expressed in the maxilla and limb and weakly expressed in the mandible and frontal primordia. mx, maxillary process; md, mandibular process. I–P, in situ hybridization of Taf4b mRNA in sections of Tgfbr2fl/fl and Tgfbr2fl/fl;Wnt1-Cre mice at E14.5. Taf4b was strongly expressed by osteoprogenitor cells in the skull, frontal bone, mandible, and maxilla of wild-type mice, whereas the gene expression of Taf4b was significantly reduced in Tgfbr2fl/fl;Wnt1-Cre mice. Boxed areas in I and L are magnified in J and M, respectively. Wild-type maxilla of each developmental stage is shown in O and P. Arrowheads point to expression of Taf4b mRNA. Open arrowheads indicate areas negative for Taf4b expression. Tg is tongue. Q, quantitative RT-PCR analyses of Taf4b from E14.5 skull, mandible (Mand), and maxilla (Max) of Tgfbr2fl/fl (open columns) and Tgfbr2fl/fl;Wnt1-Cre (closed columns) mice. *, p < 0.05. R–T, Tgf-β2 or bovine serum albumin (BSA) bead implantation experiment in maxillas from Tgfbr2fl/fl (S) and Tgfbr2fl/fl;Wnt1-Cre (T) mice at E13.5. R is a schematic diagram of the experiment design. Arrowheads indicate the expression of Taf4b mRNA detected by whole-mount in situ hybridization. Dotted line outlines the edge of palates. U, immunoblotting analysis of Taf1 in Tgfbr2fl/fl and Tgfbr2fl/fl;Wnt1-Cre maxilla at E12.5, E13.5, and E14.5. Data shown are representative of three separate experiments. V, plot shows the ratios between Taf1 and GAPDH after quantitative densitometry of immunoblotting data in U. *, p < 0.05. Tgfbr2fl/fl (white bars) and Tgfbr2fl/fl;Wnt1-Cre (black bars). W and X, immunohistochemical staining of Taf1 in sections of Tgfbr2fl/fl (W) and Tgfbr2fl/fl;Wnt1-Cre (X) mice at E14.0. Taf1 expression was significantly reduced in Tgfbr2fl/fl;Wnt1-Cre mice. Arrows point to expression of Taf1.