FIGURE 2.

AR L701H, AR L701M, and AR L701Q display modified ligand specificity. A, transcriptional activation of wild-type (WT) AR, the AR L701H and AR T877A single mutants, and the AR L701H/T877A double mutant by DHT (100 nm), progesterone (100 nm), and cortisol (1 μm). Activation of wild-type AR by DHT was set to 100%. Error bars represent mean relative luciferase activities of three independent experiments performed in duplicate (±S.E.). Protein expression of these AR mutants is shown in Fig. 1B. B, screening of AR Leu⁷⁰¹ mutants for responses to progesterone (100 nm) and cortisol (1 μm). Error bars represent mean relative luciferase activities of three independent experiments performed in duplicate (±S.E.). Transcriptional activation of wild-type AR by DHT was set to 100%.