FIGURE 4.

Dose-response curves of wild-type AR (A), AR L701M (B), AR L701H (C), and AR L701Q (D) with the panel of steroids. Hep3B cells were transiently transfected with expression vectors encoding the different AR mutants together with the (ARE)₂TATA-Luc reporter plasmid. Cells were incubated for 24 h with the indicated concentrations of hormone. For each receptor, the transcriptional activity in response to 100 nm DHT was set to 100%. Data represent the mean of three independent experiments performed in duplicate (±S.E.). wt, wild-type.