Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Dec 14;285(7):5106–5116. doi: 10.1074/jbc.M109.070995

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Effects of Mys knockdown on PKA activity and amount of inactive PKA in zebrafish embryos. A, immunoblotting of extracts from wild-type embryos (−) and embryos injected with 5mm MO (5mm) and mys MO (mys). B, PKA activity in the embryos in A (mean ± S.D., n = 3; asterisk, p < 0.001, Student's t test). Knockdown of Mys by injection of mys MO resulted in reduction of PKA activity. C, immunoblotting of extracts from wild-type embryos (−) and embryos injected with mys SD-MO (SD). D, PKA activity in the embryos in C (mean ± S.D., n = 3; asterisk, p < 0.005, Student's t test). E, immunoprecipitations of extracts from wild-type embryos (−) and embryos injected with 5mm MO (5mm) and mys MO (mys) using anti-Prkar1a antibody. F, relative amount of Prkac coimmunoprecipitated with Prkar1a in the embryos in E (mean ± S.D., n = 3; asterisk, p < 0.01, Student's t test). Knockdown of Mys increased the amount of Prkac binding to Prkar1a. G, immunoblotting of extracts from wild-type embryos (−) and embryos injected with prkar1a MO (prkar1a). H, PKA activity in the embryos in G (mean ± S.D., n = 3; asterisk, p < 0.001, Student's t test). Knockdown of Prkar1a significantly increased PKA activity.