Effects of Mys knockdown and ectopic expression of Shh and dnPKA on patterning of gene expression in the eyes, neural tube, and somites. A and B′, whole mount in situ hybridization with probes for pax2a (A–D), pax6a (E–H), foxa (I–P), ptc1 (Q–T), eng1a (U–X), and twist2 (Y–B′). Dorsal (A–H) and lateral views (I–B′) of embryos injected with 5mm MO (A, E, I, M, Q, U, and Y), mys MO (B, F, J, N, R, V, and Z), shh mRNA (C, G, K, O, S, W, and A′), and dnPKA mRNA (D, H, L, P, T, X, and B′). Embryos fixed at 26 (A–X) and 20 hpf (Y–B′). Knockdown of Mys altered gene expression patterns in the eyes, neural tube, and somites (B, F, J, N, R, V, and Z), which is similar to those in embryos ectopically expressing Shh (C, G, K, O, S, W, and A′) and dnPKA (D, H, L, P, T, X, and B′). Arrows indicate the expanded region of pax2a expression (B–D) and foxa expression (J–L). os, optic stalk; fp, floor plate; sc, sclerotome.