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. 2009 Dec 14;285(7):5106–5116. doi: 10.1074/jbc.M109.070995

FIGURE 7.

FIGURE 7.

Involvement of Mys in antagonizing Hh signaling pathway downstream of Smo. A–L, in situ hybridization with probes for pax2a (A–C), foxa (D–F), ptc1 (G–I), and eng1a (J–L). Dorsal (A–C) and lateral views (D–L) of embryos injected with 5mm MO (A, B, D, E, G, H, J, and K) and mys MO (C, F, I, and L) followed by ethanol (A, D, G, and J) or cyclopamine treatment (B, C, E, F, H, I, K, and L). Embryos were fixed at 26 hpf. Cyclopamine treatment induced significant reductions of pax2a expression in the eyes (B), foxa expression in the neural tube (E, arrow), ptc1 expression in cells adjacent to the notochord (H), and eng1a expression in somites (K). In contrast, knockdown of Mys resulted in increased expression of pax2a in the eyes (C) and ptc1 expression in somites (I), partial rescue of foxa expression in the neural tube (F, arrowhead) and rescue of eng1a expression in the somites (L) of the embryos incubated with cyclopamine.