FIGURE 8.

Effects of PKA activation on the patterning of gene expression in the eyes, neural tube, and somites in mys MO-injected embryos. A–X, in situ hybridization with probes for pax2a (A–D), pax6a (E–H), foxa (I–L), ptc1 (M–P), eng1a (Q–T), and twist2 (U–X). Dorsal (A–H) and lateral views (I–X) of embryos fixed at 26 (A–T) and 20 hpf (U–X). Treatment with dimethyl sulfoxide (DMSO) did not affect gene expression patterning of control 5mm MO-injected embryos (A, E, I, M, Q, and U) and mys MO-injected embryos (B, F, J, N, R, and V). In contrast, treatment of the embryos with forskolin rescued gene expression patterns (C, G, K, O, S, and W). In addition, co-injection of prkar1a MO with mys MO resulted in rescue of gene expression patterns (D, H, L, P, T, and X). Arrows indicate the expanded region of pax2 (B) and foxa (J) expression.