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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Anal Biochem. 2010 Jan 4;399(2):230–236. doi: 10.1016/j.ab.2009.12.037

Figure 5. The read-out system efficiently detects different levels of ADAR2 activity in a cellular environment.

Figure 5

A). Increasing concentrations of ADAR2, but not ADAR1, result in increased luciferase activity. Different concentrations of an ADAR1 or ADAR2 –expressing plasmid was transfected together with the reporter system. After 48h, cells were lysed and analyzed for luciferase expression. The experiments were repeated twice in duplicate and the mean +/− SD of all data points is shown. The values in the graph are depicted as percentage of co-transfection of the reporter system with an empty vector, which was set to 100%. B). Overall sequence trace of the hairpin cDNA after co-transfection with ADAR2. In addition, the sequences of several individual clones are shown. Cells were transfected with the reporter system in combination with an ADAR2-expressing vector. After 48 h, RNA was isolated from the cells, reverse transcribed, and either directly sequenced or subcloned followed by sequencing.