Figure 7. SOCS-1 and SOCS-3 negatively regulate IFN-β-induced macrophage and CD4+ T-cell chemotaxis.
(A)Astrocytes transfected as in Figure 6A were treated with medium (UN) or IFN-β for 16 or 24 h, and their supernatants used in a chemotactic assay with bone marrow derived primary macrophages in a Transwell migration chamber. Data are the mean ± S.D. of three experiments. *P ≤ 0.05. (B) The same supernatants were used in a chemotactic assay with spleen CD4+ T-cells in a Transwell migration chamber. Data are the mean ± S.D. of three experiments. *P ≤ 0.05 and **P ≤ 0.001.