Fig. 5.

Effects of Rem C-terminal mutants on Ca²⁺ channel current expression. Representative Ba²⁺ current elicited by a voltage step from –80 to 10 mV in HEK293 cells transiently transfected with expression vectors encoding Ca_V1.2, β_2a, and either GFP-Rem^Stop282 (A) or GFP-Rem^Stop265 (B). (Bars = 10 pA/pF and 50 ms.) (C) HEK293 cells transiently cotransfected with expression vectors encoding HA-tagged Rem or HA-Rem^Stop265 and either Flag-Ca_Vβ_2a or empty Flag vector. Whole-cell extracts were immunoprecipitated with anti-FLAG antisera and analyzed by immunoblotting as described in Fig. 4. Data are representative of a typical experiment performed three independent times.