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mRNA levels for mfs in immature and fully expanded leaves of WT and transgenic peppermint plants that overexpress (HMF) or cosuppress (LMF) mfs. Total leaf RNA was resolved on an agarose-formaldehyde gel (5 μg per lane), blotted, hybridized to the radiolabeled mfs cDNA as probe, and exposed to x-ray film (Upper). (Lower) Ribosomal RNA bands visualized with ethidium bromide that were used to verify RNA loading before transfer.
