Fig. 2.

Trolox treatment restores the mitochondrial membrane potential in complex-I-deficient patient fibroblasts. Mean TMRM pixel intensity in healthy (C-5120; black bars) and patient (S4-5260, S7-5175; open bars) fibroblasts cultured for 72 h in the absence or presence of Trolox (300 µM). In each experiment, the mean value obtained with vehicle-treated healthy fibroblasts was set at 100%, to which all values were related. (a) Significantly different from vehicle-treated C-5120. (b) Significantly different from vehicle-treated S4-5260. (c) Significantly different from vehicle-treated S7-5175. The values presented are the mean ± SEM of 100–200 individual cells. (***p < 0.001)