Table 1.
Characteristics of 16S rRNA gene libraries constructed from midgut communities in cabbage white butterfly larvae reared
| Experiment | Library name | Diet | Date of sampling | Sample composition | No. of sequences | No. of OTUs observeda | Chao estimate OTUs (95% confidence interval) | Good's coverage | Shannon–Weaver diversity index (95% confidence interval) |
|---|---|---|---|---|---|---|---|---|---|
| Effect of diet | BL-1 | Artificial | 1/05 | Pool—10 guts | 79 | 7 | 7.4 (7.0–20) | 97% | 1.2 (0.9–1.4) |
| BL-2 | Sinigrin | 1/05 | Pool—10 guts | 59 | 9 | 10 (9.1–20) | 93% | 1.7 (1.4–1.9) | |
| BL-3 | Brussels sprouts | 1/05 | Pool—10 guts | 69 | 7 | 7.3 (7.0–16) | 97% | 0.7 (0.4–1.0) | |
| Effects of antibiotics | BL-4 | Artificial | 11/05 | Pool—10 guts | 99 | 11 | 14 (11–34) | 96% | 1.7 (1.6–1.9) |
| BL-5 | PenStrep | 11/05 | Pool—10 guts | 100 | 15 | 43 (22–120) | 93% | 2.0 (1.8–2.2) | |
| BL-6 | PenStrep » artificial | 11/05 | Pool—10 guts | 98 | 10 | 13 (10–33) | 97% | 1.4 (1.2–1.6) | |
| Larva-to-larva variation | BL-7 | Artificial | 12/05 | Individual gut | 69 | 9 | 38 (18–115) | 94% | 0.8 (0.4–1.1) |
| BL-8 | Artificial | 12/05 | Individual gut | 48 | 6 | 9.0 (6.4–31) | 98% | 0.9 (0.6–1.2) | |
| BL-9 | Artificial | 12/05 | Individual gut | 56 | 8 | 16 (10–52) | 98% | 0.9 (0.6–1.3) | |
| Batch-to-batch variation | BL-10 | Artificial | 3/04 | Pool—10 guts | 136 | 6 | 7.0 (6.1–20) | 99% | 0.9 (0.6–1.1) |
| BL-11 | Artificial | 11/04 | Pool—10 guts | 146 | 9 | 9.5 (9.0–17) | 99% | 0.6 (0.5–0.8) | |
| Larva-to-larva variation | BL-12 | Artificial | 8/06 | Individual gut | 77 | 8 | 8.0 (8.0–8.0) | 99% | 1.6 (1.4–1.8) |
| BL-13 | Artificial | 8/06 | Individual gut | 82 | 8 | 14 (12–33) | 96% | 1.4 (1.2–1.6) | |
| BL-14 | Artificial | 8/06 | Individual gut | 76 | 10 | 14 (11–34) | 95% | 1.7 (1.5–1.9) |
Artificial sterile artificial diet, Sinigrin sterile artificial diet amended with sinigrin, PenStrep sterile artificial diet amended with penicillin and streptomycin, PenStrep » artificial PenStrep transferred to sterile artificial diet
aAll sequences within each OTU differ by a sequence divergence of 3% or less