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. 2009 Dec 3;86(1):103–112. doi: 10.1093/cvr/cvp384

Figure 2.

Figure 2

Resveratrol-mediated autophagy induces cell survival. H9c2 cells [1 × 104 in a 96-well plate for (A and B) and 3 × 105 in a 10 cm dish for (C and D)] were cultured as mentioned in Figure 1. (A) MTT cell survival assay was performed with MTT Cell Proliferation Assay kit (Cayman Chemical). (B) The release of lactate dehydrogenase (LDH) as a marker of cell death was performed with LDH Cytotoxicity Assay kit (Cayman Chemical). Western immunoblotting (C) and its quantification (D) with QuantiOne® software were performed with H9c2 cell lysate for the expression of cell survival marker. Figures are representative images of at least three different samples, and each experiment was repeated at least thrice. Results are expressed as mean ± SEM. *P < 0.05 vs. Normal, Resv 1, and Rapa + Resv 1; #P < 0.05 vs. HR.