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. 2009 Dec 15;86(1):82–91. doi: 10.1093/cvr/cvp396

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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2009. For permissions please email: journals.permissions@oxfordjournals.org.

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The results of mathematically modelling our experimental data, acquired from control hearts and hearts perfused with ETZ. (A) Results from the model which best-fit our experimental data from Figures 4A and 5A. Experimental data were best reproduced with a 10-fold catalytic activity of CA. (B) The model simulation of pR, based on the time courses from (A). With moderate levels of CA activity, as may be expected in the heart, the H¹³CO₃⁻/¹³CO₂ ratio indicates a steady-state pR that closely approximates the physiological values. However, with lower CA activity, the model reproduced our experimental finding of pH_i underestimation. (C) The relationship between CA activity and steady-state pR, in the presence (dashed grey) and absence (black solid) of HCO₃⁻ efflux or (D) influx. CA activity has a significant effect on the size of the pR–pH_i discrepancy, but HCO₃⁻ transport has a much smaller impact on the discordance. (E) Relationship between steady-state pR and pHi, simulated for different levels of CA activity. (F) Relationship between equilibration time and pH_i, simulated for different levels of CA activity. Equilibration time was estimated as the time taken for pR to approach steady-state pR within 0.05 U.