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. 2009 Dec 14;86(1):55–62. doi: 10.1093/cvr/cvp399

Figure 4.

Figure 4

Bacterial SMase reversibly activated ICl,swell. (A) I–V relationships for Cl− current elicited by SMase (0.03 U/mL, 15−18 min) and inhibition by DCPIB (10 μM). (B) SMase increased Cl− current by 1.1 ± 0.1 pA/pF at 60 mV (n = 30), and DCPIB (10 or 30 μM) suppressed 78 ± 6% (n = 7) or 81 ± 6% (n = 4), respectively (P < 0.01 for both). (C) Effect of SMase reversed on washout (18−20 min, n = 3, P < 0.05). (D and E) Exposure to SMase (20 min) generated endogenous long-chain ceramides and depleted a substantial fraction of sarcolemmal sphingomyelins (n = 6; *P < 0.05). For ceramides and sphingomyelins, each lipid species was compared with control using a three-way ANOVA based on two separate experimental data sets, each analysed in triplicate.