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PDGF stimulates activation of caveolae MAP kinase in vivo. Normal human fibroblasts were incubated for the indicated time in the presence of PDGF(BB) at 30 ng/ml. At the end of the incubation, caveolae membranes and cytosol fractions were prepared. Caveolae membrane (5 μg per lane) and cytosol (25 μg per lane) were separated by SDS/PAGE on 10% gels and immunoblotted with either anti-activated MAP kinase IgG (AK) or anti-MAP kinase IgG (Erk).
