Figure 5.

Expression of apoptotic markers in SKOV-3 cells after HNTMB chelator treatment. SKOV-3 cells were treated with HMTNB (0, 0.2, 0.8 μM) for 9, 24 or 48 h. PAGE and Western blot analysis of cell lysates were carried out as described (Materials and Methods). A representative experiment is shown. Immunoblotting was carried out with primary antibodies against PARP, caspase-3, caspase-7, caspase-8, caspase-9, Bcl-2, Bid, and XIAP. As an internal standard for equal loading blots were probed with an anti-β-Actin antibody.