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. 2010 Feb 25;10:72. doi: 10.1186/1471-2407-10-72

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Copyright ©2010 Kim et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HNTMB causes G2-Phase cell cycle arrest in SKOV-3 cells. SKOV-3 cells were treated with 0.3 or 0.6 μM HNTMB for 48 h. Cell cycle analysis of treated and untreated cells was carried out as described (Materials and Methods). Data were obtained by FACS analysis based on propidium-iodide intercalation into cellular chromatin and are presented as (A) relative fluorescence intensity in a 2-dimensional FACS profile (ModFit LT software; black lines = data line and model fit line of entire population; shaded areas = model components/subpopulations of G0/G1, S, G2/M, apoptotic cells or as (B) a table. Standardized gating was used for all samples. Ten thousand events were analyzed for each sample.