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. 2010 Mar 11;5(3):e9657. doi: 10.1371/journal.pone.0009657

Figure 7. The der(X)t(X;17) translocation is selected in tumors-derived from WI-38THP-1 cells harboring the GSE56 and H-RasV12 constructs.

Figure 7

(A) The WI-38THP-1 cells that express the GSE56 and oncogenic H-RasV12 exhibit genomic instability. Spectral karyotyping (SKY) analysis of WI-38THP-1 cells which were introduced with GSE56 and oncogenic H-RasV12 reveals genomic instability manifested by polysomy and several genomic aberrations. (B) A SKY analysis of WI-38THP-1 tumor-derived cells is shown, the der(X)t(X;17) translocation is circled. (C) A FISH analysis on WI-38THP-1 tumor-derived cells that harbor a shRNA against p53 (shp53) and oncogenic H-RasV12. The probe detecting chr17q24.3 (RP11-387O17 - red) is visible on two copies of chr17 and on one copy of chrX. A probe detecting the most telomeric X chromosomal region (RP11-26A4) is marked in green.