Table 1. Geometric mean titers of antibodies.
| Group | Mouse | IgG titer (ELISA) | PRNT titer | Day of death | ||||
| day 6 | day 9 | day 12 | day 6 | day 9 | day 12 | |||
| control | 1 | 1 | 247 | 2560 | 1 | 6 | 1289 | |
| 2 | 1 | 200 | n.a. | 1 | 125 | n.a. | 14 | |
| 3 | 1 | 224 | 1453 | 1 | 207 | 274 | 13 | |
| 4 | 1 | 246 | n.a. | 1 | 73 | n.a. | 11 | |
| 5 | 1 | 100 | 1495 | 1 | 91 | 221 | 15 | |
| MVA i.v. | 1 | 50 | 1694 | 7125 | 1 | 1875 | 3774 | |
| 2 | 50 | 704 | 3282 | 1 | 485 | 1493 | ||
| 3 | 50 | 1640 | 5538 | 6 | 1056 | 3112 | ||
| 4 | 50 | 1288 | 6885 | 1 | 963 | 3508 | ||
| 5 | 50 | 2863 | 8524 | 6 | 1662 | 3403 | ||
| MVA i.n. | 1 | 1 | 239 | 1642 | 1 | 161 | 462 | 17 |
| 2 | 1 | 241 | n.a. | 1 | 106 | n.a. | 9 | |
| 3 | 1 | n.a. | 1015 | 1 | n.a. | 535 | 16 | |
| 4 | 1 | 100 | 644 | 1 | 91 | 236 | 23 | |
| 5 | 1 | 268 | n.a. | 1 | 307 | n.a. | 11 |
C57BL/6 mice were infected with 3×104 TCID50 of ECTV intranasally. Mice were left untreated or immunized three days later with 5×107 TCID50 of MVA either intravenously or intranasally. Anti-MVA IgG titers were calculated by linear regression and defined as the serum dilution that resulted in an optical density of 0.30. Sera with OD value below 0.3 set to titer of 1 (negative). Geometric mean of neutralizing antibody titers by PRNT were determined as serum dilution able to neutralize 50% of the virus. Similar results were obtained in a separate experiment.
n.a. not applicable (mouse was dead or bleeding was not possible).