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. 2010 Mar 11;5(3):e9655. doi: 10.1371/journal.pone.0009655

Figure 4. Synapse Loss in DD Axons of tba-1(ju89) Animals.

Figure 4

The DD neuron axon marker Pflp-13::GFP was coexpressed with a presynaptic marker Punc-25 mCherry::RAB-3 in wild-type (A, C, E) and mutant (B, D, F) tba-1(ju89) animals (see Methods). Axon extension was similar in both the wild-type (A) and mutant (B) axons depicted. mCherry::RAB-3 puncta were reduced or missing in the tba-1(ju89) mutant axon (D, F) compared to the wild-type worm (C, E). The bracket in (F) delineates a region in which mCherry::RAB-3 puncta are missing or greatly reduced in size in the mutant. Scale bar 20 µM.