Table 4.
Immunohistochemical analysis of pre-transplant tumor specimens for topo-1 and other polypeptides implicated in topotecan resistance
| Pt. # | TTP (months) |
Topo I intensity |
MIB-1 (% +)a |
p53 intensity |
Bcl-2 intensity |
Mcl-1 intensity |
Bcl-xL intensity |
Bax intensity |
|---|---|---|---|---|---|---|---|---|
| 1 | 18.1 | 2 | 8* | 0 | 0 | 2 | 3 | 3 |
| 2 | 28.5 | 3 | 13 | 3 | 3 | 1 | 3 | 3 |
| 3 | 5.3 | 2 | 33 | 3 | 1 | 1 | 3 | 3 |
| 4 | 8.7 | 3 | 6 | 2.5 | 0 | 1 | 3 | 3 |
| 5 | 5.9 | 3 | 45 | 3 | 0 | 1 | 2 | 3 |
| 8 | 4.3 | 0 | 40 | 3 | 0 | 1.5 | 3 | 3 |
| 9 | 7.1 | 0 | 5 | 0 | 1 | 1 | 3 | 3 |
| 10 | 11.6 | 0 | 25 | 3 | 0 | 1 | 3 | 3 |
| 11 | 5.0 | 2 | 0 | 3 | 0 | 1 | 3 | 3 |
| 12 | 21.4 | 2 | 45 | 3 | 1 | 3 | 3 | 3 |
| 13 | 2.2 | 0.5 | 0 | 0 | 1.5 | 3 | 3 | 3 |
| 14 | 13.2 | 2.5 | 90 | 2.5 | 0 | 0 | 3 | 3 |
| 15 | 13.4 | 2.5 | 43 | 3 | 0 | 1 | 3 | 3 |
| 16 | 4.3 | 1 | 0 | 0 | 0 | 1.5 | 3 | 3 |
a
Estimated percentage of cells staining positive with MIB-1 antibody. All other values represent mean staining intensity in tumor cells on a scale of 0 to 3++, with values representing the mean of intensity scores from 3 or, in some cases, 2 separate cores on the same tissue microarray.