Table 1.
Primers and product sizes for the 13 exon gene CYBB.
| Primer Name | Sequencea (5′-3′) | Product Size (bps) |
|---|---|---|
| Ex1F | AGAAGCATAGTATAGAAGAAAGGC | 180 |
| Ex1R | CCCGAGAAGTCAGAGAATTTATAAC | |
| Ex2F | CTACTGTGGAAATGCGGA | 214 |
| Ex2R | AGCCAATATTGCATGGGAT | |
| Ex3F | GGACAGGGCATATTCTGTG | 249 |
| Ex3R | GCCTTTGAAAATTAGAGGAACTTAGTA | |
| Ex4F | CTTTCCTGTTAACAATTACTATTCCAT | 202 |
| Ex4R | TCCCTGGTTCCAAGTTTTCTTTAATA | |
| Ex5F | TCATACCCTTCATTCTCTTTGTTT | 281 |
| Ex5R | AGTCCTCAATTGTAATGGCCTA | |
| Ex6F | TGTGTGTGTGTGTGTTTATATTTTAC | 309 |
| Ex6R | CTGCCTAGAAATTGAGGGAC | |
| Ex7F | TTAATTTCCTATTACTAAATGATCTGGACTT | 255 |
| Ex7R | TGTCAGTAATGAAACTGTAATAACAAC | |
| Ex8F | CCTCTGAATATTTTGTTATCTATTACCACTTA | 252 |
| Ex8R | ACTTGTCCATGATATAGTTAGACAC | |
| Ex9F | GGCAAGTATTTAGGAAAAATGTCAT | 401 |
| Ex9R | GCTATTTAGTGCCATTTTTCCTG | |
| Ex10F | GAGCAAGACATCTCTGTAACT | 276 |
| Ex10R | CTCTAAGGCCCTCCGAT | |
| Ex11F | AGGGCCTGCCAAATATAAT | 274 |
| Ex11R | CTGTACACTATGGGAAGGACC | |
| Ex12F | GTATGTGCTTTTACAGAATGTCTC | 260 |
| Ex12R | GCAGATGCAAGCCTCAA | |
| Ex13F | ATCCCAAAGCTTGAAATTGTC | 251 |
| Ex13R | CATTTGGCAGCACAACC | |
| amel-F | CCCTGGGCTCTGTAAAGAATAGTG | 106 (X) |
| amel-R | ATCAGAGCTTAAACTGGGAAGCTG | 112 (Y) |
a
The following M13 sequencing tails were added: 5′-ACGACGTTGTAAAACGAC-3′ to each forward and 5′-CAGGAAACAGCTATGACC-3′ to each reverse primer.