Fig. 21.
The influence of different methods of binding the OV-TL16 antibody and its Fab′ fragment to HPMA copolymer–drug (adriamycin {ADR} or meso chlorin e6 mono(N-2-aminoethylamide) {Mce6}] conjugates on the affinity of conjugates to an ovarian carcinoma OVCAR-3 cell associated antigen. Affinity constants Ka (M−1×10−8) are shown for free antibody; antibody bound via amino groups, antibody bound via oxidized saccharide units in the hinge region; and Fab′ fragment bound via thioether bonds [181].
